Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENVFDYEDIQLIPAKCIVNSRSECDTSVRLGGH-TFKLPVVPANMQTIIDEKLAISLAENGYFYVMHR-FEPETRIDFIKDMN-ARGLFSSISVGVKDEEYEFVRQLAEENLTPEYVTIDIAHGHSNAVIEMIQHLKKHLPDSFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASK---PIIADGGIRTHGDIAKSIRFGATMVMIGSLFAGHEESPGQTIEKDGKLYKEY--FGSASEFQKGEKKNVEGKKMHVAHKGSIKDTLIEMEQDLQSSISYAGGTKLNAIRNVDYVIVKNSIFNGDKY 4FO4 Chain:B ((14-334)) ----LTFDDVLLVPAHSTVLPNT-ADLRTRLTKNIALNIPMVSASMDTVTEARLAIALAQEGGIGFIHKNMSIEQQAAQVHQVKISGGLRVGAAVGAAPGNEERVKALVEAGV--DVLLIDSSHGHSEGVLQRIRETRAAYPHLEIIGGNVATAEGARALIEAGVSAVKVGIGPGSICTTRIVTGVGV--PQITAIADAAGVANEYGIPVIADGGIRFSGDISKAIAAGASCVMVGSMFAGTEEAPGEVILYQGRSYKAYRGMGSLGAMS---KLVPEGIEGRIAYKGHLKEIIHQQMGGLRSCMGLTGSATVEDLRT----------------

General information: TITO was launched using: RESULT: Template: 4FO4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1846 -2833 -1.53 -9.51 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -1.53 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_4FO4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FO4-query.scw PDB file : Tito_Scwrl_4FO4.pdb: