Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------MKKVRKSFVLLLTGLLAVLILSAC---GQKTQQDIVAGLDE----KAKEYTSYKAK-------------------------AKMTIETGSE------PQVYNVE-----IWHKKPSLYRVYLE-----NPKKDQNQVILRNEN-------GVFVLTPSLNKSFRFQSDWPNNSSQVY-LFESLVKDVQNDSD-----AVFTAKEKKYVFETKTNYQHNKM---LPTQEITFNKKDMSPSSVKVMDTDRKVMVKVEFSSFEFNKQFDKESFDEKKNMTLSQMDVATSAKPSDTFAVKTPLELPLGVKLLEEKDISTEDGKRIIMTY---------GGEKSFTLIQEKAQIAKASSSVTLNGEPVNLGYTIGALSDASLSWTYDGVDYLLSSKDLSKEEMVTVAKSMQGQSSK 4O7O Chain:A ((6-454)) TLATKLPWSDWLSRQRWYAGRNRELATVKPGVVVALRHNLDLVLVDVTYTDGATERYQVLVGWDKAAIGVADDRTGFDALYDVAGPQFLLSLIVSSAVCGTSTGEVTFTREPDVELPFAAQPRVCDAEQSNTSVIFDRRAILKVFRRVSSGINPDIELNRVLTRAGNPHVARLLGAYQFGDALAYALGMVTEYEANAAEGWAMATASVRDLFAEGDLYAHEVGGDFAGESYRLGEAVASVHATLADSLGTAQATFPVDRMLARLSSTVAVVPELREYAPTIEQQFQKLAAEAITVQRVHGDLHLGQVLRT--PESWLLIDFEGEPGQPLDERRAPDSPLRDVAGVLRSFEYAAYGPLVDQATDKQLAARAREWVERNRAAFCDGYAVASGIDPRDSALLLGAYELDKAVYETGYETRHRPGWLPIPLRSIARLTA

General information: TITO was launched using: RESULT: Template: 4O7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1425 -28364 -19.90 -84.42 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -19.90 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.100

(partial model without unconserved sides chains): PDB file : Tito_4O7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O7O-query.scw PDB file : Tito_Scwrl_4O7O.pdb: