Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQVYIIHGYRASS--TNHWFPWLKKRLLAD-GVQADILNMPNPLQPRLEDWLDTLSLYQHTLHENTYLVAHSLGCPAILRFLEHLQLRKQLGGIILVSGFAKSLPTLQ-MLDEFTQGSFDHQKIIESAKHRAVIASK-DDQIVPFSFSKDLAQQIDAALYEVQHGGHFLEDEGFTSLPIVYDVLTSYFSKETR 2QS9 Chain:A ((4-183)) -PSKAVIVPGNGGGDVTTHGWYGWVKKELEKIPGFQCLAKNMPDPITARESIWLPFMETELH-CDEKTIIIGHSSGAIAAMRYAETH----RVYAIVLVSAYTSDLGDENERASGYFTRPWQWEKI-KANCPYIVQFGSTDDPFLPWKEQQEVADRLETKLHKFTDCGHFQNTEF----HELITVVKSLLK----

General information: TITO was launched using: RESULT: Template: 2QS9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 951 -147142 -154.72 -840.81 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -154.72 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_2QS9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QS9-query.scw PDB file : Tito_Scwrl_2QS9.pdb: