Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVHYGLKGITCVETSISHIDGEKGRLIYRGHHAKDIALNHSFEEAAYLILFGKLPSTEELQVFKDKLAAERNLPEHIERLIQSLPNNMDDMSVLRTVVSALGENTYTFHPKTEEAIRLIAITPSIIAYRKRWTRGEQAIAPSSQYGHVENYYYMLTGEQPSEAKKKALETYMILATEHGMNASTFSARVTLSTESDLVSAVTAALGTMKGPLHGGAPSAVTKMLEDIGEKEHAEAYLKEKLEKGERLMGFGHRVYKTKDPRAEALRQKAEEVAGNDRDLDLALHVEAEAIRLLEIYKPGRKLYTNVEFYAAAVMRAIDFDDELFTPTFSASRMVGWCAHVLEQAENNMIFRPSAQYTGAIPEEVLS 2P2W Chain:A ((2-358)) -IQKGLEGVKICESSICYLDGINGRLYYRGIPVEELAEKSTFEETAYFLWYGKLPTKSELEEFKRKMADYRELPAEALGILYHLPKNLHYIDVLKIFLSIH-------EDLREKAIRVASVFPTILAYYYRYSKGKELIRPRKDLSHVENFYYMMFGER--NEKIRLLESAFILLMEQDINASTFAALVIASTLSDLYSCIVGALGALKGPLHGGASEKVPPMLEEIGSEDRVEEFVQKCLKEKRKIMGFGHRVYKTYDPRAVFLKRVLQEHFPDSKLFRIASKLEEYIVSN-----KIKNIYPNVDLYSSVLFEELGFPRNMFTALFATARVVGWTAHVIEYVSDNKLIRPTSEYVGPMDVEYI-

General information: TITO was launched using: RESULT: Template: 2P2W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1735 -216782 -124.95 -619.38 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -124.95 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_2P2W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P2W-query.scw PDB file : Tito_Scwrl_2P2W.pdb: