Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAERMVGKQAPRFEMEAVLASKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMSDRYDEFEDLDAEVIGVSTDTIHTHLAWINTDRKENGLGQLKYPLAADTNHEVSREYGVLIEEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVLQALQ----TGGLCPANWKPGQKTL 3SBC Chain:E ((25-200)) -----VQKQAPTFKKTAVV-DGVFDEVSLDK--YKGKYVVLAFIPLAFTFVSPTEIIAFSEAAKKFEEQGAQVLFASTDSEYSLLAWTNIPRKEGGLGPINIPLLADTNHSLSRDYGVLIEEEGVALRGLFIIDPKGVIRHITINDLPVGRNVDEALRLVEAFQWTDKNGTVLPCNWTPGAATI

General information: TITO was launched using: RESULT: Template: 3SBC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 898 -120072 -133.71 -698.09 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain E : 0.83 3D Compatibility (PKB) : -133.71 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.611

(partial model without unconserved sides chains): PDB file : Tito_3SBC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SBC-query.scw PDB file : Tito_Scwrl_3SBC.pdb: