Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNKLTSFLADRKKTIENQLSVYTEKLD-MPDSLKKSMLYSLQAGGKRLRPLIVLAVLNAYGKSEKDGIPVGCAVEMIHTYSLIHDDLPCMDDDDLRRGKPTNHKVFGEATAVLAGDGLLTESFKLITSHVSDEVSAEKRLRLVNELISAAGTEGMVGGQVADMEAENRQVTLEELESIHERKTAKLLGFCVIAGAILA-DAPEEDIETLRTFSSHIGIGFQIRDDILDLEGSEEKIGKRVGSDTTNDKSTYPSLLSLEGAKHKLDVHIKEAKRLIGGLSLQKDLLYELCDLIAARDH 3TS7 Chain:A ((7-301)) --RSLSDFMRSSQERVERALDARLPAADRMPERLHQAMRYSVLGGGKRMRPLLTYATGQTIGVAADLLDGPACAVEFIHVYSLIHDDLPAMDDDDLRRGKPTCHKAYDEATAILAGDGLQALAFHVLAQDPSIAVPAENRIAMIETLAKASGPAGMVGGQAIDLASVGKKLDLPGLENMHIRKTGALIRASVRLACLARPGLPAEQFDRLDHYAKCIGLAFQIQDDILDEESD---------------KPNYPALLGLSGAKEKAEEMHEAALESLAGFGPEADLLRELARFIIQRQ-

General information: TITO was launched using: RESULT: Template: 3TS7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1344 -28232 -21.01 -101.55 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -21.01 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_3TS7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TS7-query.scw PDB file : Tito_Scwrl_3TS7.pdb: