Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSKLSILMIGFALSVLLAACGSNDAKEEKTDTGSKTEATASEGEELY-QQSCVGCHGKDLEGVSGPNLQEVGGKY-----DEHKIESIIKNGR------GNMPKGLVDD-NEAAVIAKWLSEKK 2EXV Chain:A ((1-82)) -----------------------------------------EDPEVLAKNKGCVACHAIDTKM-VGPAYKDVAAKFAGQAGAEAELAQRIKNGSQGVWGPIPMPPNA-VSDDEAQTLAKWVLSQK

General information: TITO was launched using: RESULT: Template: 2EXV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 166 9114 54.90 132.09 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 54.90 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_2EXV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EXV-query.scw PDB file : Tito_Scwrl_2EXV.pdb: