Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIAVIGTGYVGLVSGTCFAEIGN--KVVCCDIDESKIRSLKNGVIPIYEPGLADLVEKNVLDQRLTFTNDIPSAIRASDIIYIAVGTP-----MSKTGEADLTYVKAAAKTIGEHLNGYKVIVNKSTVPVGTGKLVQSIVQKASKGRYSFDVVSNPEFLREGSAIHDTMNMERAVIGSTS----HKAAAIIEELHQPF--HAPVIKTNLESAEMIKYAANAFLATKISFINDIANICERVGADVSKVADGVGLDSRIGRKFLKAGIGFGGSCFPKDTTALLQIAKSAGYP--FKLIEAVIETNEKQRVHIVDKLL-TVMGSVKGRTISVLGLAFKPNTNDVRSAPALDIIPMLQQLGAHVKAYDPIAIPEASAIL---------GEQVEYYT----DVYAAMEDTDACLILTDWPEVKEMELVKV-KTLLKQPVIIDGRNLFS--LEEMQAAGYIYHSIGRPAVRGTEPSDKYFPGLPLEELAKDLGSVNL 3ITK Chain:E ((5-465)) IKKICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPTLPIYEPGLKEVVE-SCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQNSNGYKIVTEKSAVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDRVLIGGDETPEGQRAVQALCAVYEHWVPREKILTTNTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVIDMNDYQRRRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDP-KVPREQIVVDLSHPGVSEDDQVSRLVTISKDPYEACDGAHAVVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDGLHNELQTIGFQIETIGK------------------------------

General information: TITO was launched using: RESULT: Template: 3ITK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 2179 -57088 -26.20 -133.07 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain E : 0.81 3D Compatibility (PKB) : -26.20 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.568

(partial model without unconserved sides chains): PDB file : Tito_3ITK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ITK-query.scw PDB file : Tito_Scwrl_3ITK.pdb: