Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMEKTFIMVKPDGVQRQLIGDILSRFERKGLQLAGAKLMRVTEQMAEKHYAEHQGKPFFGELVEFITSGPVFAMVWEGENVIEVTRQLIGKTNPKEALPGTIRGDYGMFVGKNIIHGSDSLESAEREINIFFKNEELVSYQQLMAGWIY 2HVE Chain:A ((4-151)) -CERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGGDSVESAEKEIGLWFHPEELVDYTSCAQNWIY

General information: TITO was launched using: RESULT: Template: 2HVE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 663 -71629 -108.04 -483.98 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -108.04 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_2HVE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HVE-query.scw PDB file : Tito_Scwrl_2HVE.pdb: