Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENVFDYEDIQLIPAKCIVNSRSECDTSVRLGGH-TFKLPVVPANMQTIIDEKLAISLAENGYFYVMHR----------------FE-PETRIDFIKDMNARGLFSSISVGVKDEEYEFVRQLAEENLTPEYVTIDIAHGHSNAVIEMIQHLKKHLPDSFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASK---PIIADGGIRTHGDIAKSIRFGATMVMIGSLFAGHEESPGQTIEKDGKLYKEY-FGSASEFQKGEKKNVEGKKMHVAHKGSIKDTLIEMEQDLQSSISYAGGTKLNAIRNVDYVIVKNSIFNGDKY 4FEZ Chain:A ((14-356)) ----LTFDDVLLVPAHSTVLPNT-ADLRTRLTKNIALNIPMVSASMDTVTEARLAIALAQEGGIGFIHKNMSIEQQAAQVHQVKIFES---KPNACKDEQGR-LRVGAAVGAAPGNEERVKALVEAGV--DVLLIDSSHGHSEGVLQRIRETRAAYPHLEIIGGNVATAEGARALIEAGVSAVKVGIGPGSICTTRIVTGVGV--PQITAIADAAGVANEYGIPVIADGGIRFSGDISKAIAAGASCVMVGSMFAGTEEAPGEVILYQGRSYKAYR---------------------IAYKGHLKEIIHQQMGGLRSCMGLTGSATVEDLRT----------------

General information: TITO was launched using: RESULT: Template: 4FEZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1625 22905 14.10 82.99 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 14.10 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_4FEZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FEZ-query.scw PDB file : Tito_Scwrl_4FEZ.pdb: