Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKAKLGKSDLQVFPIGLGTNAVGGHNLYPNLNEETGKELVREAIRNGVTMLDTAYIYGIGRSEELIGEVLREFNREDVVIATKAAHRKQGNDFVFDNSPDFLKKSVDESLKRLNTDYIDLFYIHFPDEHTPKDEAVNALNEMKKAGKIRSIGVSNFSLEQLKEANKDGLVDVLQGEYNLLNREAEKTFFPYTKEHNISFIPYFPLVSGLLAGKYTEDTTFPEGDLRNEQEHFKGERFKENIRKVNKLAPIAEKH--NVDIPHIVLAWYLARPEIDILIPGAKRADQLIDNIKTAD-VTLSQEDISFIDKLFA 1YNP Chain:A ((21-307)) MKKRQLGTSDLHVSELGFGCMSLGT-------DETKARRIMDEVLELGINYLDTADLYNQGLNEQFVGKALKGR-RQDIILATKV-------------SKAYIKEAVKDSLRRLQTDYIDLYQLHGGTIDDPIDETIEAFEELKQEGVIRYYGISSIRPNVIKEYLKRSNIVSIMMQYSILDRRPE-EWFPLIQEHGVSVVVRGPVARGLLSRRPLPEGEG------------------YLNYRYDELKLLRESLPTDRPLHELALQYCLAHDVVATVAAGASSIDQVKANVQAVEATPLTAEERQHIQKLA-

General information: TITO was launched using: RESULT: Template: 1YNP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1427 -139882 -98.03 -520.01 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -98.03 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_1YNP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YNP-query.scw PDB file : Tito_Scwrl_1YNP.pdb: