Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIGIFGGTFDPPHNGHLLMANEVLYQAGLDEIWFMPNQIPPHKQNEDYTDSFHRVEMLKLAIQSNPSFKLELVEMEREGPSYTFDTVSLLKQRY-PNDQLFFIIGADMIEYLPKWYKLDELLNLIQFIGVKRPGFH-V--ET---------------------PYPLLFADVPEFEVSSTMIRERFKSKKPTDYLIPDKVKKYVEENGLYES 1K4M Chain:A ((2-213)) KSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPPHRPQ-PEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPLAFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPEDLHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR-

General information: TITO was launched using: RESULT: Template: 1K4M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 890 -45294 -50.89 -242.21 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -50.89 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_1K4M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1K4M-query.scw PDB file : Tito_Scwrl_1K4M.pdb: