Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVITYGTFDLLHWGHIKLLERAKQLGDYLVVAISTDEFNLQKQKKAYHSYEHRKLILETIRYVDEVIPEKNWEQKKQDIIDHNIDVFVMGDDWEGKFDFLKD-----QC--EVVYLPRT-EGISTTKIKEEIAGL 3GLV Chain:A ((2-122)) MIRVMATGVFDILHLGHIHYLKESKKLGDELVVVVARDSTARNNGKIPIFDENSRLALISELKVVDRAILGHE-GDMMKTVIEVKPDIITLGYDQKFDEAELQSKINKLGITVKIVRISKYD---------------

General information: TITO was launched using: RESULT: Template: 3GLV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 472 -54956 -116.43 -486.34 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -116.43 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_3GLV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GLV-query.scw PDB file : Tito_Scwrl_3GLV.pdb: