Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAGILGVGRYIPEKVLTNHDLEKM-VETSDEWIRTRTGIEERRIAA-DDVFS----SHMAVAAAKNALEQAEVAAEDLDMILVATVTPDQSFPTVSCMIQEQLGAKKACAMDISAACAGFMYGVVTGKQFIESGTYKHVLVVGVEKLSSITDW-EDRNTAVLFGDGAGAAVVGPVSDDRGILSFELGADGTGGQHLYLNEKRHT-----------IMNGREVFKFAVRQMGESCVNVIEKAGLS-KEDVDFLIPHQANIRIMEAARERLELPVEKM--SKTVHKYGNTSAASIPISLVEEL--EAGKIKDGDVVVMVGFGGGLTWGAIAIRWGR- 1UB7 Chain:A ((2-322)) --SGILALGAYVPERVMTNADFEAYL-DTSDEWIVTRTGIKERRVAAE-----DEYTSDLAFKAVEDLLRRHPGALEGVDAVIVATNTPDALFPDTAALVQARFGLK-AFAYDLLAGCPGWIYALAQAHALVEAGLAQKVLAVGAEALSKIIDWN-DRATAVLFGDGGGAAVVGKVREGYGFRSFVLGADGTGAKELYHACVAPRLPDGTSMKNRLYMNGREVFKFAVRVMNTATLEAIEKAGL-TPEDIRLFVPHQANLRIIDAARERLGLPWER-VA-VNVDRYGNTSTASIPLALKEAVDA--GRIREGDHVLLVSFGAGLTWAAAVLTWGGA

General information: TITO was launched using: RESULT: Template: 1UB7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1949 -221456 -113.63 -745.64 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -113.63 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_1UB7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UB7-query.scw PDB file : Tito_Scwrl_1UB7.pdb: