Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKGMFFCARAVVPFMKKSKDGAIVNVGSIAGITGAGSSMPYAVSKSAVHGLTKSLAHALAPEIRVSGVAPGAVAT---RWWAGRE--------EKMKSMIG-SLLLQCIAEPDDVAKLICSLIEQES--LTGQIITIDSGQTL 2DTX Chain:A ((108-248)) --GYYYASKFAIPYMIRSRDPSIVNISSVQASIITKNASAYVTSKHAVIGLTKSIALDYAPLLRCNAVCPATIDTPLVRKAAELEVGSDPMRIEKKISEWGHEHPMQRIGKPQEVASAVAFLASREASFITGTCLYVDGGLSI

General information: TITO was launched using: RESULT: Template: 2DTX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 403 -30817 -76.47 -242.65 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -76.47 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.387

(partial model without unconserved sides chains): PDB file : Tito_2DTX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DTX-query.scw PDB file : Tito_Scwrl_2DTX.pdb: