Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNVAIVGDGGHGKVIRELINARSDTRLAAVLDDKFKTFEGGKEWYTGPPKAVTELRRLIP--DVLFLIAVGNNSVRKQLAERLGL-GKDDFITLIHPSAIVSKSAVIGEGTVIMAGAIIQADARIGAHCIINTGAVAEHDNQISDYVHLSPRATLSGAVSVQEGAHVGTGASVIPQIIIGAWSIVGAGSAVIRSIPDRVTAAGAPARIISSIQTSNKG 4EA9 Chain:A ((12-217)) IGGVVIIGGGGHAKVVIESLRACG-ETVAAIVDADP--AVLGVPVVGDDLA----LPMLREQGLSRLFVAIGDNRLRQKLGRKA-RDHGFSLVNAIHPSAVVSPSVRLGEGVAVMAGVAINADSWIGDLAIINTGAVVDHDCRLGAACHLGPASALAGGVSVGERAFLGVGARVIPGVTIGADTIVGAGGVVVRDLPDSVLAIGVPAKIKG--------

General information: TITO was launched using: RESULT: Template: 4EA9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1254 -87979 -70.16 -439.90 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -70.16 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_4EA9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EA9-query.scw PDB file : Tito_Scwrl_4EA9.pdb: