Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRILSILVFAIMLAGCSSNASTEKQHAGG-----EKTVKAEPQSTSSQKDSTDDYQPNSQVTDDRTLHKVGQTFSDDKGKAVLKDIKQV-NKTYKIGDVELTVKEMKLIHLRPD------YSMIDYFHELTHDEEFDFVKVFVDIKNTSTKKVNVAPIALMKTNTGETF------------DWNKDIYLEELNGELEGGAEKSGNLGFIVNASSGHAHDKAADAEKKTKEIKWIEITTSDVFDHNHKKISDAQKIKIKF- 5F18 Chain:A ((12-232)) ----------------PSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGAD-------VPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQN-------SHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKN----------YKNPNLTISFT

General information: TITO was launched using: RESULT: Template: 5F18.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 796 -39579 -49.72 -201.93 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -49.72 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.291

(partial model without unconserved sides chains): PDB file : Tito_5F18.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F18-query.scw PDB file : Tito_Scwrl_5F18.pdb: