Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEDFLLSNGYQLGKTIGEGTYSKVKEAFSKKHQRKVAIKVIDK-MGGPEEFIQRFLPRELQIVRTLDHKNIIQVYEMLESADGKICLVMELAEGGDVFDCVLNGGPLPESRAKALFRQMVEAIRYCHGCGVAHRDLKCENALLQGFN----LKLTDFGFAKVLPKSHRELSQTFCGSTAYAAPEVLQGIPHDSKKGDVWSMGVVLYVMLCASLPFDDTDIPKMLWQQQKG---VSFPTHLSISADCQDLLKRLLEPDMILRPSIEEVSWHPWLAST 4FG8 Chain:A ((20-277)) ---------YDFRDVLGTGAFSEVILAEDKRTQKLVAIKCIAKE---------N----EIAVLHKIKHPNIVALDDIYESG-GHLYLIMQLVSGGELFDRIVEKGFYTERDASRLIFQVLDAVKYLHDLGIVHRDLKPENLLYYSLDEDSKIMISDF-------------------TPGYVAPEVLAQKPY-SKAVDCWSIGVIAYILLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWIAGD

General information: TITO was launched using: RESULT: Template: 4FG8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -144419 for 1666 contacts (-86.7/contact) + 2D Compatibility (PS) -23791 + (NN) -11521 + (LL) 1960 1D Compatibility (HY) -21200 + (ID) 4150 Total energy: -203121.0 ( -121.92 by residue) QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_4FG8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FG8-query.scw PDB file : Tito_Scwrl_4FG8.pdb: