Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQIRTIDKQ-RLTDKITHLDDEMMDKVDEALQISLALIDF 1UB4 Chain:A ((5-111)) YVPDMGDLIWVDFDP-------GHRPAVVLSPFMYNNKTGMCLCVPCTTQSK--GYPFEVVLS----GQERDGVALADQVKSIAWRARGATKKGTVAPEELQLIKAKINVLIG----

General information: TITO was launched using: RESULT: Template: 1UB4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 478 -71477 -149.53 -721.98 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -149.53 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_1UB4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UB4-query.scw PDB file : Tito_Scwrl_1UB4.pdb: