Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMQKQVKISGKSKENMSLLKHLKGDVQGKELVIEDSIVNERWKQVLKEKIDIEHDLFNYQKNREISKVPFLPVDRLITNDEVEDILNTLTEVLPTGKFTSGPYLEQFEKVLSTYLHKRYVIATSSGTDAIMIGLLALGLNPGDEVIMPANSFSATENAVLASGGVPIYVDINPQTFCIDPDKIEEAITPYTKFILPVHLYGKHSDMQHIRQIANRYKLKVIEDACQGIGLTDL----GKYADITTLSFNPYKNFGVCGKAGAIATDNEELAKKCIQFSYHGFEVNV-KNKKVINFGFNSKMDNLQAAIGLERMKYLSLNNFKRLFLADRYITQLAELQ----NKGYIELPE-LSEDHVWHLFPIKVRTE----DRADIMTKLNEDFGVQTDVYYPILSHMQKTPLVQDKYAGLQLVHTEKAHSQVLHLPLYPSFTLEEQDRVMEGLFHVIKQEIGV
4LC3 Chain:A ((24-390))-------------------------------------------------------------------------------IDEETIQGVVEVLRSGWITTGPQCQKFEAALSEYCGGRPVRVFNSGTCTLEIGLRIAGVGPGDEVITTPASWVSTSNVIIETGATPVFADIDPVTRNIDLDKLEQAITPRTKAIIPVFLSGLPVDMDRLYAIARAHKLRVIEDAAQAFGSTWHGKRIGAIGDLVSFSFHANKNLTTI-EGGALVLNNEDEAVLAQKYRLQGITRTGFDGMDCDVLGGKYNLTDVAARVGLGQLPHLERFTAQRRALARAYFAAFDGGAAAKLG---VGLPVAEFENGNWHMFLVTLPLERLTITRAEFMAQMKE-RGIGTGIHYP-AIHLFTLY-RARGFKEGMFPHAERYGASTVTLPLFTQMTEGDVRRVVDAVNQICEQYG--


General information:
TITO was launched using:
RESULT:

Template: 4LC3.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1998 -203768 -101.99 -577.25
target 2D structure prediction score : 0.68
Monomeric hydrophicity matching model chain A : 0.70

3D Compatibility (PKB) : -101.99
2D Compatibility (Sec. Struct. Predict.) : 0.68
1D Compatibility (Hydrophobicity) : 0.70
QMean score : 0.519

(partial model without unconserved sides chains):
PDB file : Tito_4LC3.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-4LC3-query.scw
PDB file : Tito_Scwrl_4LC3.pdb: