Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVKKVVTHPAEVLETPAETVTVFDKKLKKLLDDMYDTMLEMDGVGLAAPQIGILKRAAVVEIGDDR--------GRIDLVNPEILEKSGEQTGI-EGCLSFPNVYGDVTRADYVKVRAFNRQGKPFILEARGFLARAVQHEMDHLDGVLFTSKISKYYTEDELADMEG 3U04 Chain:A ((3-172)) MSVLSIVTVPDKRLSLCSEEVEKVDQSIRKLVDDMFETMHANQGLGLAAVQVGVHKRILVMNVPEE-IEGYELYGGPYCIINPKIVDISQEKVKLKEGCLSVPGYFDYIVRPQRIAVQYLDYNGNECIIKAQGWLARCLQHEIDHLNGTVFLKYLSKF-----------

General information: TITO was launched using: RESULT: Template: 3U04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 753 -89228 -118.50 -602.89 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -118.50 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_3U04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U04-query.scw PDB file : Tito_Scwrl_3U04.pdb: