Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHLQLKKLLNHFFLEDIGTGDLTSQSIFGEQSCEAEIVAKSEGIFAGAAIIKEGFSLLDENVQSILHKKDGDMLHKGE------VIAELHGPAAALLSGERVVLNLIQRLSGIATMTREAVRCLDDEQIK--ICDTRKTTPGLRMLEKYAVRAGGGYNHRFGLYDGIMIKDNHIAACGSILEACKKARQAAGHMVNIEVEIETEEQLREAIAAGADVIMFDNCPPDTVRHFAKLTPANIK---TEASGGITLESLPAFKGTGVNYISLGFLTHSVKSLDISMDVTLSNESVEECCYVNS 3C2F Chain:A ((13-290)) -----WRQDVTNWLSEDVPSFDFGGY-VVGSDLKEANLYCKQDGMLCGVPFAQEVFNQCELQVEWLF--KEGSFLEPSKNDSGKIVVAKITGPAKNILLAERTALNILSRSSGIATASHKIISLARSTGYKGTIAGTRKTTPGLRRLEKYSMLVGGCDTHRYDLSSMVMLKDNHIWATGSITNAVKNARAVCGFAVKIEVECLSEDEATEAIEAGADVIMLDN------------------HFLLECSGGLNL------LCDDIDIYSTSSIHQGTPVIDFSL-----------------

General information: TITO was launched using: RESULT: Template: 3C2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1100 -10183 -9.26 -42.43 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -9.26 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_3C2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C2F-query.scw PDB file : Tito_Scwrl_3C2F.pdb: