Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSELWYTEKQTKNFGITMKVNKTLHTEQTEFQHLEMVETEEFGNMLFLDGMVMTSEKDEFVYHEMVAHVPLFTHPNPEHVLVVGGGDGGVIREILKHPSVKKATLVDIDGKVIEYSKKFLPSI-AGKLDDPRVDVQVDDGFMHIAKSENQYDVIMVDSTEPVG---PAVNLFTKGFYAGIAKALKEDGIFVAQTDNPWFTPELITN-VQRDVKEIFPITKLYTANIPTYPSGLWTFTIGSKKYDPLAVEDS------RFFDIETKYYTKDIHKAAFVLPKFVSDLIK 3ANX Chain:A ((5-280)) ---MYFFEHVTPYETLVRRMERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKRVLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDDARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFYRLVKAHLNPGGVMGMQAGMILLTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLN-FGFLLASDAFDPAAFSEGVIEARIRERNLALRHLTAPYLEAMFVLPK-------

General information: TITO was launched using: RESULT: Template: 3ANX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1472 -55458 -37.67 -209.27 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -37.67 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_3ANX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ANX-query.scw PDB file : Tito_Scwrl_3ANX.pdb: