Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------MTQQYIVEPKKGLGLKLKKGQILKVVDVEGQQVAD-----FVAYHAKDFYEHLDQGATIDANHS-------IHVKV-----NDHLYSNLYKPMLTLIEDTVGKHDLLLPACRPDMNRLLYGKQKDEFQDTCYDNMNRALEQFGVPKPHMHY-PFAIFMNTVLDEKGNLSVETPLSNAGDYVRLRAEMDLIVAFSSCPIEKGKCNGDS------VTSIRVEVS-------------- 4TQ0 Chain:A ((4-273)) DKDVLRDVWFGRIPTCFTLYQEAEPYYLLLPRVSYLTLVTDKVKKHFQKVMEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQ--FWAINRKLMEYPAEENGFRYIPFRIYQTTT-ERPFIQKLFRPVAADGQLHTLGDLLKEVC--PSAIKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQP

General information: TITO was launched using: RESULT: Template: 4TQ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 723 -66034 -91.33 -342.15 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -91.33 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.120

(partial model without unconserved sides chains): PDB file : Tito_4TQ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TQ0-query.scw PDB file : Tito_Scwrl_4TQ0.pdb: