Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINSIQWEYIFNTKLAIESFPYVIKGIGYTLLISFVSMFAGTVIGLFIS-LARMSKLALLRWPAKLY------ISFMRGVPILVILFILYFGFPYIGIEFS-AVTAALIGFSLNSAAYIAEINRSAISSVEKGQWEAASSLGLSYWQTMRGIILPQSIRIALPPLANVLLDLIKASSLAAMITVPELLQHAKIIGGREFDYMTMYILTALIYWAICSIAAVFQNILEKKYAHYV 3TUJ Chain:A ((10-148)) -----------------------VRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPGQIIANAKLYRTVSAIVNIFRSIP-FIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPE----ALPGLVN-------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3TUJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 398 -72013 -180.94 -549.72 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -180.94 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.104

(partial model without unconserved sides chains): PDB file : Tito_3TUJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TUJ-query.scw PDB file : Tito_Scwrl_3TUJ.pdb: