Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKYEIIANEMRNRIKNNVYPIDQPIPDEVSLAKEFNSSRMTMKRALDNLVAEGLLFRKRGHGTFIIQSAIQDDHVHVVSNEILGLTNLLK-DKKIKSKVIQFEVQFPTEEVAAHLSIDQKTPVYYVVRLRIVEGEPYVLEKTYMPTHLIPGINDDVLHDSIYNHITNVLQLKIAGTHR--------KIRACKSDHIDQQHLGCKQDDPILEVEHVGFL--DTGIPFEYSFSRHRHDKFVVTSVNIRR 3EDP Chain:A ((11-233)) ---FEVIASKIKDSINRDEYKTG--MPNETALQEIYSSSRTTIRRAVDLLVEEGLVVRKNGVGLYV--------QPKLTAQNILEMTGVMKN---LKKDIKDFYIRKAGKFYAEIFGMKENELVYSIKFVQKSE-HGATLDRLILPLGLYPDLQAKDF------QIINIIELVNSGKYKLFELEQELQLILAGNEQIKNMHLN--ENDPVFKLSSVFYAENDMPIAIQYHYEDAESTKYVV-------

General information: TITO was launched using: RESULT: Template: 3EDP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 942 -91480 -97.11 -446.24 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -97.11 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_3EDP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EDP-query.scw PDB file : Tito_Scwrl_3EDP.pdb: