Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIDYVSDLHINHWIPWNV-------NQIKWEKRTREIVNRLISNGNGEVLVIAGDFTEWNQ--------QTLWVLDEAAKQYEKVYFTYGNHDLYLLSKSQKRKYSDSLGRLNDLIQKAADMKNV-TPLIK-TTET---YKGKVFAGDVMWYLPKGIEGWDFFKGVSNDSNY----------------IWLNGYNKVDGVRAMWKESMDW-YETLE----NTQVDVFVSHVPPVHNPYSPFEP-N--TCYMVDVPFI-NAKHWVCGHDHLQAEFDK-DGTSFHMNCIGYPYDYDNYPSVNVIPGEEVDSYKTFELKTFEI 2NXF Chain:A ((6-285)) FTFGLIADVQYADIEDGENYLRTRRRYYRGSADLLRDAVLQWR-RERVQCVVQLGDIIDGHNRRRDASDRALDTVMAELDACSVDVHHVWGNHEFYNFSRPS---------LLSSRLNSAQ---GSDLIGDDIYAYEFSPAPNFRFVLLDAYDLSVIGREEESEK-HTHSWRILTQHNHNLQDLNLPPVSVGLEQRFVKFNGGFSEQQLQWLDAVLTLSDHKQERVLIFSHLPVHPCAADPICLAWNHEAVLSVLRSHQSVLCFIAGHDHDGGRCTDSSGAQHITLEG---------------------------------

General information: TITO was launched using: RESULT: Template: 2NXF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1077 6756 6.27 29.63 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 6.27 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.265

(partial model without unconserved sides chains): PDB file : Tito_2NXF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NXF-query.scw PDB file : Tito_Scwrl_2NXF.pdb: