Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVKKLVVVSMLSSIAFVLMLL-NFPFPGLPDYLKIDFSDVPAIIAILIYGPLAGIAVEAIKNVLQYIIQGSMAGVPVGQVANFIAGTLFILPTAFLFKK-LNSAKGLAVSLLLGTAAMTILMSILNYVLILPAYTWFLHSPALSDSALKTAVVAGILPFNMIKGIVITVVFSLIFIKLKPWIEQQRSAHIH 4HZU Chain:S ((4-164)) GSLKENTIAAVLIAMTVALSILVVIPIPA--TKGIVTLCEVGIYTSAILYGRRMGLLVGGASGFLIDILTGY----PVWCLFSLVIHGTQGLVVGWLLPRHHKGIRSMLLPLLVGSLVMVIGYCLATTLL----F----G---------WP-AGLASIFGNVVQVGFGAGVTLSIVGPLTRLKPD-------

General information: TITO was launched using: RESULT: Template: 4HZU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain S - contact count / total energy / energy per contact / energy per residue : 715 -135258 -189.17 -850.68 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain S : 0.68 3D Compatibility (PKB) : -189.17 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.322

(partial model without unconserved sides chains): PDB file : Tito_4HZU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HZU-query.scw PDB file : Tito_Scwrl_4HZU.pdb: