Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSILFIFGLILGSFYYTAGCRIPLHLSIIAPRSSCPFCRRTLTPAELIPILSFLFQKGKCKSCGHRISFMYPAAELVTACLFAAAGIRFGISLELFPAVVFISLLIIVAVTDIHFMLIPNRILIFFLPFLAAARLISPLDSWYAGLLGAAAGFLFLAVIAAITHGGVGGGDIKLFAVIGFVLGVKMLAAAFFFSVLIGALYGAAAVLTGRLAKRQPLPFAPAIAAGSILAYLYGDSIISFYIKMALG 5FLM Chain:L ((15-43)) -------------------------------MIYICGECHTENEIKS--------RDPIRCRECGYRI------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FLM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 77 -4997 -64.89 -172.29 target 2D structure prediction score : 0.17 Monomeric hydrophicity matching model chain L : 0.33 3D Compatibility (PKB) : -64.89 2D Compatibility (Sec. Struct. Predict.) : 0.17 1D Compatibility (Hydrophobicity) : 0.33 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_5FLM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FLM-query.scw PDB file : Tito_Scwrl_5FLM.pdb: