Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYDHILIRFGEISTKGKNRKSFIERLKQNIRLVLKDYPNLKYFSNRDRMTITLNGEDPEALFPHLKQVFGIQSFSLAIKCDSRLDDIKATALKAIKDQY--KPGDTFKVATKRAYKQFELDTNQMNAEIGGHILRNTEGLTVDVRNPDIPLRIEIREEATFLTIRDEKGAGGLPVGSAGKAMLMLSGGFDSPVAGFYAMKRGLSVEAVHFFSPPYTSERAKQKVMDLAKCLSRFG--GSMTLHIVPFTKTQELIQKQIPENYTMTATRRLMLQIADRIREKRNGLAIITGESLGQVASQTLESMYAINAVTSTPILRPLIAMDKTEIIEKSREIGTYETSIQPFED-CCTIFTPPSPKTRPKKEKIEHFESFV-DFEPYIQEAVDNIETMTLYSEQEANDKFAELF 4KR6 Chain:A ((3-387)) -ELRVYIVRYSEIGLKGKNRKDFEEALRRNIERVTG-M---KVKRQWGRFLIPIDE--NVTLDDKLKKIFGIQNFSKGFLVSHDFEEVKKYSLIAVKEKLEKGNYRTFKVQAKKAYKEYKKGVYEINSELGALILKNFKELSVDVRNPDFVLGVEVRPEGVLIFTDRVECYGGLPVGTGGKAVLLLSGGIDSPVAGWYALKRGVLIESVTFVSPPFTSEGAVEKVRDILRVLREFSGGHPLRLHIVNLTKLQLEVKKRVPDKYSLIMYRRSMFRIAEKIAEETGAVAFYTGENIGQVASQTLENLWSIESVTTRPVIRPLSGFDKTEIVEKAKEIGTYEISIKPYQDSCVF-FAPKNPATRSHPSILEKLEQQVPDLPVLEEEAFTSRKVEVI--------------

General information: TITO was launched using: RESULT: Template: 4KR6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2080 -23512 -11.30 -62.04 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -11.30 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_4KR6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KR6-query.scw PDB file : Tito_Scwrl_4KR6.pdb: