Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENFIGSHMIYTYENGWEYEIYIKNDHTIDYRIHSGMVAGRWVRDQEVNIVKLTEGVYKVSWTEPTGTDVSLNFMPNEKRMHGIIFFPKWVHEHPEITVCYQNDHIDLMKESREKYETYPKYVVPEFAEITFLKNEGVDNEEVISKAPYEGMTDDIRAGRL 3NX1 Chain:A ((9-158)) LSGFVGKHLVYTYDNGWNYEIYVKNDNTIDYRIHSGLVGNRWVKDQEAYIVRVGESIYKISWTEPTGTDVSLIVNLGDSLFHGTIFFPRWVMNNPEPTVCFQNDHIPLMNSYREAGPAYPTEVIDEFATITFVRDCGANNESVIACAASE-----------

General information: TITO was launched using: RESULT: Template: 3NX1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 760 -75413 -99.23 -502.75 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -99.23 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_3NX1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NX1-query.scw PDB file : Tito_Scwrl_3NX1.pdb: