Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIIYDYERGIENNVALTFGNFDGIHLGHELAISTLKKIAQERGFPSAILTFEPHPSTILFNRNNFRLINQEQKKELISSYGIDYLYIINFNKGFSQISCDDFISKILIDRY--NTKHIIVGESCTFGHKRLGNTLILRKHSETYGYSLTKLEPLTVNGKICSSSSIREHIQKGEIEIANKLLGRSYQVSGTVMKGALRGREIGFPTINIPI-EDCMIKPKFGTYYAKVAFTSLDVIPARDAGIYRSQYQSNETTEGSAKGSFSGSGPSWLYGVVNIGMRPTFNDLKKPIVEMHIFDFNEDIYDRKVNIQLLKFIRSEKRFLSVDELTKQINHDILEAYQLRTDL 1S4M Chain:A ((1-284)) --------------MVVSIGVFDGVHIGHQKVLRTMKEIAFFRKDDSLIYTISYPPEYFLPDFPGL-LMTVESRVEMLSRYA--RTVVLDF------FRIKDLTPEGFVERYLSGVSAVVVGRDFRFGKNASGNASFLRKK----GVEVYEIEDVVVQGKRVSSSLIRNLVQEGRVEEIPAYLGRYFEIEGIVHK---------FPTANIDRGNEKLVDLKRGVYLVRVHL-----------------------PDGKKK-----------FGVMNVGFRPT--DARNVKYEVYILDFEGDLYGQRLKLEVLKFMRDEKKFDSIEELKAAIDQDVKSARNMIDDI

General information: TITO was launched using: RESULT: Template: 1S4M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -186253 for 2080 contacts (-89.5/contact) + 2D Compatibility (PS) -29138 + (NN) -5479 + (LL) 2780 1D Compatibility (HY) -25200 + (ID) 4650 Total energy: -247940.0 ( -119.20 by residue) QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_1S4M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S4M-query.scw PDB file : Tito_Scwrl_1S4M.pdb: