Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNDQIVFEKTKNIAHDINQMQNQQEIIDYLFRQDSLTLNQLKHYYSEPSLPLQFLVKVAVLCMFISMTLASFLFIQAKEVFTNTILSDISPAVFSIFTVICIFMTYTKIIKKGNKNKGKASLNQRSEFYEKNKLINTILYKKYKMDQQNIQANKHTASDNEDSMNFSAVLNHVLTISKNDKELLGYLDTRDNAMLSQLKAYFSTRPFSLPHYMSLMFCGSIIVVYATSLFSGQINYIDIPHIFIFLLLIIFLKILIDLIKLLNISRKGQLHTVLHFAQRAEYLRMRGVIDFILTERYNKKIM 2P1A Chain:A ((85-116)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MIQGYDLLSKTFLSYSNEQLAEMKTAYWGISY-----------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2P1A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 19 -1162 -61.16 -36.31 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.48 3D Compatibility (PKB) : -61.16 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.48 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_2P1A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P1A-query.scw PDB file : Tito_Scwrl_2P1A.pdb: