Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITLFQCLYLILFSFICYQGAAAFSHSTAASWLAAALGAAAAGLYIWNTKRVWKHCSSGLCAWIAVIQVMSVGVVLIGTDIMPVLCVIAIFAGCEGLRIGQSALQARLSDQIDKLTQAEQHANQMLIDVRSRNHDTMKHITAIKSAQPKADTQAYIQNWADQYSQYDRFLKGENAYVAGVLYDFLEKARASNVSVSLHMHTPLS---SLPFSPADQVSLVGNILENALDSAAEAREKAEIKLETSLRSGLYVLTCENSTPGMDPKVLDTIYQSFGRSTKNGAHEGMGTYIIQKLVKGAFGRLDFTYRHP-IFRLEIKIPFQK 1ID0 Chain:A ((1-146)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACKYCLE-----FVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAREITEQYEGKIVAGESMLGGARMEVIFGRQH

General information: TITO was launched using: RESULT: Template: 1ID0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 688 -80457 -116.94 -566.60 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -116.94 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_1ID0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ID0-query.scw PDB file : Tito_Scwrl_1ID0.pdb: