Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNKQLKKKAQEIGNVPTHYELEIEDYDQKQKKYGQAYFIWKDPKDPEKHITVELRNDGALLTFSTTVHSETDKKLPDAELKLTALQFAAANHPGTFMNFHFQGKEERGQHIRFVYTKMELGLPIPNSGFLIDMTRSGQIVHFLYYGEGHKAEVPTEFVAKEKVVSHYLNTMSFELMYDVIDGEQEPRLVYEPILPGYSYPADVDEIVPDQHIADERIENTAPLPPLQNKEEVDIFALLGFTSDMQKVSERDFGEQIGSTWRRGAAPERKDLSIGSYFETRNKNTIKMKTDKRTGKLKAALSFMD----WRNNLQCSTEECQEIALQFLYALY-PRAA--EFFRVNPV--------RIDERGR-VRNHFSVWYKGVPLRF--------------GAARIIVNPETGLIDAFMAPDIEP-EQLEAINHRPDVSAEEAKEAFLAAFD------------VKLEWQPDFTAGSDQHCKLVYKPVY------------------PSYIDAHIRKKKRL 5COZ Chain:A ((139-356)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SKFGTYYSPFNDKDKFRTDKQLMFMSAEEAEELAVKTAKELEINVCEKNELYVLDDKNTLIFPEDDTDKQNDTYVFFMFPDVYGIPYSRCPENEALTGYANQENHLVIAMDE-KG-ISFLDIPPLYDWVETT--ETGEILHPSSILSKEVDKLKKYVTSGDIEVSEISLEYMLFADKNETYDIKPVWVVYYYQNQLVTGENSYTQKMALYDVYDAYTGEEYR-

General information: TITO was launched using: RESULT: Template: 5COZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 597 -43896 -73.53 -279.59 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -73.53 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.319

(partial model without unconserved sides chains): PDB file : Tito_5COZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5COZ-query.scw PDB file : Tito_Scwrl_5COZ.pdb: