Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAKRAIPVRERNIVLIGFMGVGKTTIGQLVAKKLYRDFIDIDQQIEKDFNMSIPEIFEKKGEDFFRKTEKEYILDICHHKRFKIVSLGGGSFKQEEIRNCCLENCLVLHLD----LSWENWKQRADLLIESRPVLHNRSMDEMEQLFNERKVIYDKHNSKVATDNLSPEEVADYIVETLKIGWDLYQPM 3MUF Chain:A ((8-147)) -----------QHLVLIGFMGSGKSSLAQELGLALKLEVLDTDMIISERVGLSVREIFEELGEDNFRMFEKNLIDELKTLKTPHVISTGGGIVMHENLKG--LGTTFYLKMDFETLIKRLNQKER-----EKRPLLNN--LTQAKELFEKRQALYEKNAS------------------------------

General information: TITO was launched using: RESULT: Template: 3MUF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 577 -64047 -111.00 -470.93 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -111.00 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_3MUF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MUF-query.scw PDB file : Tito_Scwrl_3MUF.pdb: