Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAENQEVYDVTIIGGGPIGLFTAFYCGMRELKTKVIEFLPKLGGKVSLFFPEKIIRDIGGIPGIAGKQLIEQLKEQAATFDPDIVLNQRVTGFERLDDGTIVLTGSEGKKHYTRTVILACGMGTLEVNEFDSEDAARYAGKNLHY-GVEKLDAFKGKRVVISGGGDTAVDWANELEPIAASVTVVHRREEFGGMESSV-TKMKQSSVRVLTPYRLEQLNGDEEGIKSVTVCHTESGQRKDIEIDELIINHGFKIDLGPMMEWGLEIE-EGRVKADRHMRTNLPGVFVAGDAAFYESKLRLIAGGFTEGPTAVNSAKAYLDPKAENMAMYSTHHKKLVHK 3F8P Chain:A ((14-319)) -----EKFDVIIVGLGPAAYGAALYSARYMLKTLVIGETP--GGQLT---EAGIVDDYLGLIEIQASDMIKVFNKHIEKYEVPVLLDI-VEKIEN---EFVVKTKRKGE-FKADSVILGIGVKRRKLGVPGEQE---FAGRGISYCSVCDAPLFKNRVVAVIGGGDSALEGAEILSSYSTKVYLIHRRDTFKAQPIYVETVKKKPNVEFVLNSVVKEIKGDKV-VKQVVVENLKTGEIKELNVNGVFIEIGFDPPTDFAKSNGIETDTNGYIKVDEWMRTSVPGVFAAGDCTSAWLGFRQVITAVAQGAVAATSAYRYVTEK-----------------

General information: TITO was launched using: RESULT: Template: 3F8P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1839 -204897 -111.42 -682.99 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -111.42 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_3F8P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F8P-query.scw PDB file : Tito_Scwrl_3F8P.pdb: