Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKSWKPQELSISYHQFTVFQKDSTPPVMDWTDEAIEKGYAAADGAISFEAQRNTKAFILFRLNSSETVNSYEKKVTVPFHVTENGIHIESIMSKRLSFDLPKGDYQLTCWTVPAEMSDLHADTYIIDAVSV 4MQD Chain:A ((1-132)) MIKSWKPQELSISYHQFTVFQKDSTPPVMDWTDEAIEKGYAAADGAISFEAQRNTKAFILFRLNSSETVNSYEKKVTVPFHVTENGIHIESIMSKRLSFDLPKGDYQLTCWTVPAEMSDLHADTYIIDAVSV

General information: TITO was launched using: RESULT: Template: 4MQD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 679 -78749 -115.98 -596.58 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -115.98 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.736

(partial model without unconserved sides chains): PDB file : Tito_4MQD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MQD-query.scw PDB file : Tito_Scwrl_4MQD.pdb: