Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKIKYLYQLLLSHISILILAFVIIISLFSHFVKEFAYQNKVEELTSYAVQIANEFQSGQVDMRRLYPYQDILSTRKTQFIIFNEEQQPYFLPEGFHHPPREQLKKSEWNKLKKGQTVTIRADGRFDDEVSLVAQPIFVQNEFKGAVLLISPISGVEQMVNQVNLYMFYAVISTLVITILVSWLLSKFHVKRIQKLREATDKVASGDYDIHLENSYGDEIGVLASDFNIMAKKLKQSRDEIERLEKRRRQFIADVSHELKTPLTTINGLVEG-------LNSHTIPEDKKDKCFSLISEETKRMLRLVKENLDYEKIRSQQITLNKLDVPLIEVFEIVKEHLQQQAEEKQNKLMIQVEDHVIVHA--DYDRFIQILVNITKNSIQFTQNG--DIWLRGMEGYKE--TIIEIEDTGIGIPKEDIEHIWERFYKADISRTNTAYGEYGLGLSIVRQLVEMHQGTVEIKSEEGKGTKFIIRLPLTAKQQ 4JAV Chain:A ((13-246)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LKRIDRMKTEFIANISHELRTPLTAIKAYAETIYNSLGELDLSTLKE-----FLEVIIDQSNHLENLLNELLDFSRLERKSLQINREKVDLCDLVESAVNAIKEFASSHNVNVLFESNVPCPVEAYIDPTRIRQVLLNLLNNGVKYSKKDAPDKYVKVILDEKDGGVLIIVEDNGIGIPDHAKDRIFEQFYRVDSSLTYEVPG-TGLGLAITKEIVELHGGRIWVESEVGKGSRFFVWIP------

General information: TITO was launched using: RESULT: Template: 4JAV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 893 -96276 -107.81 -435.64 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -107.81 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_4JAV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JAV-query.scw PDB file : Tito_Scwrl_4JAV.pdb: