Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKWMCSICCAAVLLAGGAAQAEAVPNEPINWGFKRSVNHQPPDAGKQLNSLIEKYDAFYLGNTKEKTIYLTFDNGYENGYTPKVLDVLKKHRV-TGTFFVTGHFVKDQPQLIKRMSDEGHIIGNHSFHHPDLTTKTADQIQDELDSVNEEVYKITGKQDNLYLRPPRGVFSEYVLKETKRLGYQTVFWSVAFVDWKINNQKGKKYAYDHMIKQAHPGAIYLLHTVSRDNA----EALDDAITDLKKQGYTFKSIDDLMFEKEMRLPSL 4M1B Chain:A ((48-243)) --------------------------------------------------------VIYKGDTSKKQVAFTFDISWGDKKAIPILDTLKERDIKNATFFLSAAWAERHPDVVERIIKDGHEIGSMGYNYTSYTSLETNEIRRDLLRAQDVFTKLGVKQIKL-LRPPSGDFNKATLKIAESLGYTVVHWSNNSNDWK---NPGVNKIVSTVSNNLKGGDIVLLH--ASDSALQTNKALPLLLQKLKSDGYEQISVSQLI----------

General information: TITO was launched using: RESULT: Template: 4M1B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1025 -101493 -99.02 -531.38 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -99.02 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_4M1B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M1B-query.scw PDB file : Tito_Scwrl_4M1B.pdb: