Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIVALHVVLYQPEIPANTGNIARTCAATNTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLE-----ELFEAYKKGKFFFITKFGQQPHTSFDYTDLDEDYFFVFGRETSGLPKDLIQN-NMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLK 3N4K Chain:A ((5-158)) -----LNIVLFEPEIPPNTGNIIRLCANTGCQLHLIKPLGFTWDDKRLRRAGLDYHEFADIKHHHDYQAFLDSEKLDSTQPARLFALTTKGTPAHSAVSYQAND---YLLFGPETRGLPAYILDALPAQQKIRIPMQADSRSMNLSNAVSVVVYEAWRQLGY----

General information: TITO was launched using: RESULT: Template: 3N4K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 658 -97379 -147.99 -657.97 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -147.99 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_3N4K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N4K-query.scw PDB file : Tito_Scwrl_3N4K.pdb: