Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRREERNMDKLLISFLLSLFMVYFPPSDVVLPSQFEASTDSYVPMSSYPQETQSAKTPSPGSMHPAELIKEYSPLAQSVRQLSVKPLDEPLINRLEKALAVPVKYQSNYLRI 4ESH Chain:A ((46-83)) ------------------------------------------------------------------------TPLAERIRELLLAPSDEPMAADTELLLMFAARAQ------

General information: TITO was launched using: RESULT: Template: 4ESH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 49 -5595 -114.18 -164.56 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -114.18 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.696

(partial model without unconserved sides chains): PDB file : Tito_4ESH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ESH-query.scw PDB file : Tito_Scwrl_4ESH.pdb: