Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMMKYEAVIIGGGPVGFMLASELAIAGVGTCVIERLEKPVPHSKALTLHPRTLELLEMRGILERFVSKG--------------------SKIPTGHFSMLDTRLDFSGLDTSCPYTLLLPQSKTEKLLEDHARSLGTEVFRGAEALAVTQNGEAVQTIFKD-RDGSVRTITSKFAVGADGAGSTVRKQAKIEFPGTDSTVTAALGDVVLLSPPPSGVLSLCTKEGGVMIVPLSPDRYRVVVISP-----YRTQTPKDVPVTEEELKADLLRIC---GTDFG-LTDPSWMSRFGNAARQAKRYRDGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAAIKGSAPSWLLDSYHDERHPAAEGLLRNTEAQTK------LIDFTQAGLHLRSMMSELLAFPDVNR------------YVAGQISALDVRYEADRTMPPNRLNGARLPDMKLILSDGNSE---RLYSFLQNGTF--VLLSLRQEADDHIEVKGLRTVTASLAEPNEKLRNVHTILIRPDGHVAWAVDASAPDCSEVIQKGISRWFSVTSRV 2R0C Chain:A ((25-544)) --IETDVLILGGGPVGMALALDLAHRQVGHLVVEQTDGTITHPRVGTIGPRSMELFRRWGVAKQIRTAGWPGDHPLDAAWVTRVGGHEVYRIPLGTADTRATPEHTPEPDAICPQHWLAP------LL---AEAVGERLRTRSRLDSFEQRDDHVRATITDLRTGATRAVHARYLVACDGASSPTRKALGIDAPPRHRT--QVFRNILFRAPE---LRSLLGERAALFFFLMLSSSLRFPLRALDGRGLYRLTVGVDDASKSTMDSFELVRRAVAFDTEIEVLSDSEWHL----THRVADSFSAGRVFLTGDAAHTLSPSGGFGMNTGIGSAADLGWKLAATLRGWAGPGLLATYEEERRPVAITSLEE------RELPPGLHDDGPRGERIRAAVAEKLERSGARREFDAPGIHFGHTYRSSIVCGE---------WRPSARPGARAPHAWLTPTTSTLDLFGRGFVLLSFGTTDGVEAVTRAFADRHVP---LETVTCHAPEIHALYERAH-VLVRPDGHVAWRGDHLPAELGGLVDK------------

General information: TITO was launched using: RESULT: Template: 2R0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2316 -219850 -94.93 -490.74 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -94.93 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.359

(partial model without unconserved sides chains): PDB file : Tito_2R0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R0C-query.scw PDB file : Tito_Scwrl_2R0C.pdb: