Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIERINEHTVKFYMSYGDIEDRG---FDREEIWYNRERSEELFWEVMDEVHEEEEFAVEGPLWI---QVQALDKGLEIIVTKAQLSKDGQKLELPIPEDKKQEPASEDLDALLDDFQKEEQAVNQEEKEQKLQFVLRFGDFEDVISLSKLNVNGSKTTLYSFENRYYLYVDFCNMTDEEVENQLSILLEYATESSISIHRLEEYGKLIISEHALETIKKHFAS 3FWN Chain:A ((98-188)) ---------SLKPYLDKGDIIIDGGNTFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIAAVAEDGEPCVTYIGAD----------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3FWN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 279 -11479 -41.14 -135.05 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -41.14 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.265

(partial model without unconserved sides chains): PDB file : Tito_3FWN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FWN-query.scw PDB file : Tito_Scwrl_3FWN.pdb: