Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTIKTSNLGFPRIGLNREWKKALEAYWKGSTDKDTFLKQIDELFLSAVKTQIDQQIDVVPVSDFTQYDHVLDTAVSFNWIPKRF-RHLTDATDTYFAIARGIK----DAVSSEMTKWFNTNYHYIVPEYDESIEFRLTRN-KQLEDYRRIKQEYGVETKPVIVGPYTFVTLAKGYEPSEAKAIQ--KRLVPLYVQLLKELEEEGVKWVQIDEPALVTASSEDVRGA-KELFESITSELSSL-NVLLQTYF-DSVDAYEELISYP-VQGIGLDFVHDKGRNLEQLKT--HGFPTDKVLAAGVIDGRNIWKADLEERLDAVLDILSIAKVDELWIQPSSSLLHVPVAKHPDEHLEKDLLNGLSYAKEKLAELTALKEGLVSGKAAISEEIQQAKADIQALKQFATGANSEQKKELEQLTDKDFKRPIPFEERLALQNESLGLPLLPTTTIGSFPQSAEVRSARQKWRKAEWSDEQYQNFINAETKRWIDIQEELELDVLVHGEFERTDMVEYFGEKLAGFAFTKYAWVQSYGSRCVRPPVIYGDVEFIEPMTVKDTVYAQSLTSKHVKGMLTGPVTILNWSFPRNDISRKEIAFQIGLALRKEVKALEDAGIQIIQVDEPALREGLPL-KTRDWDEYLTWAAEAFRLTTSSVKNETQIHTHMCYSNFEDIVDTINDLDADVITIEHSRSHGGFLDYLKNHPYLKGLGLGVYDIHSPRVPSTEEMYNIIVDALAVCPTDRFWVNPDCGLKTRQQEETVAALKNMVEAAKQARAQQTQLV 4ZTY Chain:A ((2-762)) ---VQSSVLGFPRMGVLRDLKKANEAYWADKISQEALLAEGKRLRLAHWKIQKDAGVDIIPSNDFAHYDHVLDHIQLFNAVPERYTSQKLSPLDEYFAMGRGHQKGGVDVPALEMVKWFDSNYHYVKPTLQDNQTFSLAKDPKPVREFLEAK-EAGFQTRPVLVGPVSFLALGKADRGSSVDPITLLDKLVPVYVELLKQLKAAGAESVQIDEPVLVFDLRPEVKAAFKPAYEAIAAAGDAVPKVVVATYFGDIVHNFDVLPAFSGAAGLHVDLV----RNPEQLEPVLKQLGPNQILSAGVVDGRNIWKNDFAKSLEILQTAVKALGSERVIVATSSSLIHTPHTLASEKKLPSDVYEWFSFAVEKVKEVATLAKA-VTEPEAVKAELEANAAAIKARTDSKRTNDPAVKERQAQVTPEQHNRKAPFNTRYAEQKKHLSLPLFPTTTIGSFPQTSEIRVQRNKFTKGEISAEEYERFIEKEIELAVKIQDELDLDVYVHGEPERNDMVQYFGERLNGYVFTTHAWVQSYGSRCVRPPIIVGDISRPAPMTVKESKYAASISKKPMKGMLTGPVTCLRWSFPRVDVHQSVQCQQLALALRDEVVDLEKNGIYVIQVDEPALREGLPLRKGQEREAYLKWAVDSFKLATAGVENSTQIHSHFCYSEFQDFFHAIAALDADVLSIENSKSDAKLLKVFIDEEYPRHIGPGVYDIHSPRVPTLEEFKQRIEEMLAYLKPEQLWINPDCGLKTRKWDEVKGALSHMVEAAKYFR-------

General information: TITO was launched using: RESULT: Template: 4ZTY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4289 -305133 -71.14 -409.03 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -71.14 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_4ZTY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZTY-query.scw PDB file : Tito_Scwrl_4ZTY.pdb: