Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKAYLTVCSNQQIADRVFQMVLKGELVQGFTTPGQFLHLKVSEAVTPLLRRPISIADVNFEKNEVTIIYRVDGEGTRLLSLKQQ-GELVDVLGPLGNGFPVNEVQPGKTALLVGGGVGVPPLQELSKRLIEKGVN--VIHVLGFQSAKDVFYEEECRQYGD--------TYVATADGSYGETGFVTDVIKRKKL---EFDILLSCGPTPMLKALKQEYAHKEVYLSMEERMGCGIGACFACVCHTNESETSYVKVCLDGPVFKAQEVAL 1KRH Chain:A ((179-317)) -------------------------------------------------------------------------GKMSEYLSVQAKAGDKMSFTGPFGS-FYLRDVK--RPVLMLAGGTGIAPFLSMLQVLEQKGSEHPVRLVFGVTQDCDLVALEQLDALQQKLPWFEYRTVVAHAESQHERKGYVTGHIEYDWLNGGEVDVYL-CGPVPMVEAVR------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1KRH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 439 -85169 -194.01 -681.35 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -194.01 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_1KRH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KRH-query.scw PDB file : Tito_Scwrl_1KRH.pdb: