Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFEWNKYFPFHNQFSKEALKKADPKEVETYVNRVMESVFGSDYAAQFPFRDPLPQKEHPAKPDAKPDVKPDIDIFETADHVFVKVPISEEW--LEQVRIKHTSHELWLENLPRADHPKKVNLPCLVKRKGTKAVYKDGLLEVMFQKQQDYNMSEVEIIR 1SM9 Chain:A ((88-174)) ----------------------DPKNVETALNKTLADL-KVDYVDLFLIHFPIAFKFVPIEEKYPPGFYCG----DGNNFVYEDVPILETWKALEKLVAAGKIKSIGVSNFPGA---------------------------------------------

General information: TITO was launched using: RESULT: Template: 1SM9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 254 2369 9.32 27.86 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 9.32 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_1SM9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SM9-query.scw PDB file : Tito_Scwrl_1SM9.pdb: