Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLIKLEQPNCNPCKMVSNYLEQVNIQFE------TVDVTQEPEVAARFGVMGVPVTILLSDQGEEVNRSVGFKPNELDELLKELR 2O85 Chain:A ((19-94)) VQLVDFWATWCGTCKMIAPVLEELAADYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKD-GQPVDKVVGFQPKE---------

General information: TITO was launched using: RESULT: Template: 2O85.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 232 -177 -0.76 -2.53 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -0.76 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.675

(partial model without unconserved sides chains): PDB file : Tito_2O85.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O85-query.scw PDB file : Tito_Scwrl_2O85.pdb: