Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYESKTGNVKRFVKALQQEFDVEAIEIT----DDTIINQEFIHITYTIG----FGEVPERTLSFINKNKNK--IRGVAVSGNKVWGDNYGLAGDKLSAKFHTPLLLKFELSGTKQDLQKIIQEVQLIDKHNTKLDQAQ 3N3A Chain:C ((27-145)) --FSSSSENTQRFIERL----GLPAVRIPLNERERIQVDEPYILIVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCGVPWLYRFELMGTQSDIENVRKGV--------------

General information: TITO was launched using: RESULT: Template: 3N3A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 480 12311 25.65 112.94 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : 25.65 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_3N3A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N3A-query.scw PDB file : Tito_Scwrl_3N3A.pdb: