Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDYSQFPRKNILCVDMKSFYASVSAVTMGLNPL--TCYLAVVGNTDRQGSVVLAASPALKKDFGIKTGSRL-FEIPEDPRIHIVNPQMKLFIRVSTEITKLFYRFVPEKCVHTYSIDESFLDAGKE-----DPEEMAKAIQSSMWREFGLMCTVGIGDNMLLSKLALDLESKKTKSGIARWRYEDVPNKLWKVRPLSKMWGIGGRMERNLNRMGISTIGQLAKFPLELLEKKFGIMGNQLYYHAHGIDLSEIGAPLMQGQISFGKSQILLRDYTRSEEIKAVLLEICEEVARRARTHNKVGRTISLGIGYSKDELGGGFHRSKTIDLPTSITMDIYRCCLMLFNKFYSGKTVRSVSVTLSNIEDDVNQQLSLFEVDNEKRRKLGFVMDGIRSKYGSKAILRAVSYTPAGTALQRAGLTGGHKS 4Q45 Chain:F ((3-274)) ---------RKIIHVDMDCFFA---AVEMRDNPALRDIPIAIGGSRERRGVISTANYPARK--FGVRSAMPTGMALALCPHLTLLPGRFDAYKEASNHIREIFSRYTSR--IEPLSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQFVIT---PAEVPAFL-QTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQACDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRK--SVGVERTMAEDIHHWSECEAIIERLYPELERR----------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4Q45.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1227 -713 -0.58 -2.70 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain F : 0.68 3D Compatibility (PKB) : -0.58 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_4Q45.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q45-query.scw PDB file : Tito_Scwrl_4Q45.pdb: